Native to Brazil, Peperomia prostrata grows in warm, humid conditions where roots stay lightly moist but not trapped in dense, soggy material. That background points to a loose, airy substrate with good drainage and some organic content, so moisture is available without cutting off oxygen around the roots.
Its root system is relatively fine and shallow, which makes texture especially important. The best soil for String of Turtles feels light and open, with a sandy-loamy structure that drains well but still holds a small, steady reserve of moisture. A slightly acidic to near-neutral reaction suits its natural preferences.